Fields »

SUBCELLULAR LOCATION

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Main location(s)

Nucleus but not nucleoli, Cytoplasm, Focal Adhesions

Additional location(s)

 

Staining summary

Localized to the cytoplasm, nucleus and focal adhesion sites.

Reliability (APE)

Low

Antibodies in assay

CAB001716, HPA002199
 

Antibody CAB001716

 

Antibody HPA002199

 

         Cell line A-431

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Location(s)

Nucleus but not nucleoli, Cytoplasm Nucleus but not nucleoli, Focal Adhesions 

Staining characteristics

  

Staining intensity

Moderate Moderate 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 
One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 

Comment

  

         Cell line U-2 OS

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Location(s)

Cytoplasm Nucleus but not nucleoli, Focal Adhesions 

Staining characteristics

  

Staining intensity

Weak Moderate 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 
One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 

Comment

  

         Cell line U-251 MG

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Location(s)

Cytoplasm Nucleus but not nucleoli, Focal Adhesions 

Staining characteristics

  

Staining intensity

Moderate Weak 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 
One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 

Comment

  

         Antibody summary


Immunofluorescent staining of human cell line A-431 shows positivity in nucleus but not nucleoli & cytoplasm.
 

Immunofluorescent staining of human cell line A-431 shows positivity in nucleus but not nucleoli & focal adhesions.
 

Summary

Staining of cytoplasm in all three cell lines with additional staining of nucleus in A-431.   Staining of nucleus and focal adhesions in all three cell lines.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is partly supported by literature or no literature is available.
 

Dictionary

Cytoplasm
Focal adhesions
Nucleus but not nucleoli