Fields »

SUBCELLULAR LOCATION

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Main location(s)

Nucleus, Cytoplasm

Additional location(s)

 

Staining summary

Localized to the cytoplasm and nucleus.

Reliability (APE)

Medium

Antibodies in assay

HPA026425, CAB027391
 

Antibody HPA026425

 

Antibody CAB027391

 

         Cell line A-431

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Location(s)

Nucleus but not nucleoli, Cytoplasm Nucleus but not nucleoli, Cytoplasm 

Staining characteristics

 Granular 

Staining intensity

Moderate Moderate 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 
One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 

Comment

  

         Cell line U-2 OS

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Location(s)

Nucleus but not nucleoli, Cytoplasm Nucleus but not nucleoli, Cytoplasm 

Staining characteristics

  

Staining intensity

Moderate Moderate 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 
One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 

Comment

  

         Cell line U-251 MG

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Location(s)

Nucleus but not nucleoli Nucleus, Cytoplasm 

Staining characteristics

  

Staining intensity

Strong Moderate 

Validation

Location not consistent with experimental gene/protein characterization data, but supported by ≥1 other antibody.
 
One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 

Comment

  

         Antibody summary


Immunofluorescent staining of human cell line A-431 shows positivity in nucleus but not nucleoli & cytoplasm.
 

Immunofluorescent staining of human cell line A-431 shows positivity in nucleus but not nucleoli & cytoplasm.
 

Summary

Staining of nucleus in all three cell lines with additional staining of cytoplasm in A-431 and U-2 OS.   Staining of nucleus and cytoplasm in all three cell lines.  

Validation

The subcellular location is supported by literature.
 
The subcellular location is supported by literature.
 

Dictionary

Cytoplasm
Nucleus