Fields »

SUBCELLULAR LOCATION

? »

Main location(s)

Nucleus, Cytoplasm

Additional location(s)

 

Staining summary

Localized to nucleus and cytoplasm.

Reliability (APE)

High

Antibodies in assay

HPA002548, CAB022718
 

Antibody HPA002548

 

Antibody CAB022718

 

         Cell line A-431

Toggle channels

 
 

Location(s)

Nucleus but not nucleoli, Cytoplasm Nucleus but not nucleoli, Cytoplasm 

Staining characteristics

Granular  

Staining intensity

Moderate Weak 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 
One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 

Comment

  

         Cell line U-2 OS

Toggle channels

 
 

Location(s)

Nucleus but not nucleoli, Cytoplasm Nucleus, Cytoplasm 

Staining characteristics

Granular  

Staining intensity

Weak Weak 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 
One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 

Comment

  

         Cell line U-251 MG

Toggle channels

 
 

Location(s)

Nucleus but not nucleoli, Cytoplasm Nucleus but not nucleoli, Cytoplasm 

Staining characteristics

Granular  

Staining intensity

Moderate Weak 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 
One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 

Comment

  

         Antibody summary


Immunofluorescent staining of human cell line U-251MG shows positivity in nucleus but not nucleoli & cytoplasm.
 

Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleus & cytoplasm.
 

Summary

Staining of nucleus and cytoplasm in all three cell lines.   Staining of nucleus and cytoplasm in all three cell lines.  

Validation

The subcellular location is supported by literature.
 
The subcellular location is supported by literature.
 

Dictionary

Cytoplasm
Nucleus