The normal tissue page shows antibody staining and, when available, knowledge-based annotated protein expression in 46 human tissues. The assay and annotation is described here.
This page starts with a summary. The summary data can be based on one or more antibodies (reported by the "Antibodies in assay"-field). If the data is based on one single antibody, the IH validation score for that antibody is reported in the "Reliability (Single)"-field (color-coding described here). If the data is based on more than one antibody, the reliability score for the data is reported as "Reliability (APE)" (color-coding described here).
The tissues and cell types can be ordered by organ, cell type or alphabetically.
For each tissue and cell type, the antibody staining is given with the yellow-scale color-coding (described by the scale in the box to the right). Each available antibody is listed in a separate column and the antibody identifier is available at the bottom of the tissue list. The images and annotations can be accessed by clicking on the tissue name.
When available, a knowledge-based annotated protein expression (APE) is given to the right of the antibody staining values. The level of expression is given by a blue-scale color-coding (described by the scale in the box to the right).
At the very bottom of this page, a summary of the staining from each antibody is given, together with a representative image and the immunohistochemistry validation score for the antibody.
Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in glial cells.
Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in islet cells.
Immunohistochemical staining of human colon shows distinct positivity in microvilli of glandular cells.
Immunohistochemical staining of human fallopian tube shows distinct positivity in cilia.
Summary
Most normal tissues displayed weak to moderate cytoplasmic positivity. Glial cells in CNS, glandular cells in thyroid and myocytes showed strong cytoplasmic staining.
Goblet cells of gastrointestinal tract and islet cells of pancreas showed strong cytoplasmic immunoreactivity. Remaining normal tissues were negative.
Weak to moderate cytoplasmic positivity, with cases of nuclear or membranous staining was seen in most normal tissues. Intestinal tract displayed strong membranous staining.
Cilia in for example fallopian tube and airway epithelia showed moderate staining. Subsets of neuronal cells also dispalyed moderate cytoplasmic staining. Intercalated discs in heart were distinctly stained. Most of the remaining normal tissues were negative or displayed weak cytoplasmic positivity.
Validation
Two (or more) antibodies yielding partly similar staining patterns which are partly consistent with gene/protein characterization data or consistent with limited gene/protein characterization data
One antibody yielding a staining pattern which is partly consistent with gene/protein characterization data or consistent with limited gene/protein characterization data
Two (or more) antibodies yielding partly similar staining patterns which are partly consistent with gene/protein characterization data or consistent with limited gene/protein characterization data
Two (or more) antibodies yielding partly similar staining patterns which are partly consistent with gene/protein characterization data or consistent with limited gene/protein characterization data
