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SUBCELLULAR LOCATION

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Main location(s)

Nucleus but not nucleoli, Cytoskeleton (Microtubules)

Additional location(s)

Golgi apparatus 

Staining summary

Mainly localized to the nucleus.

Reliability (APE)

Medium

Antibodies in assay

HPA018122, HPA027478
 

Antibody HPA018122

 

Antibody HPA027478

 

         Cell line A-431

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Location(s)

Nucleus but not nucleoli Nucleus but not nucleoli 

Staining characteristics

 Granular 

Staining intensity

Strong Weak 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data.
 
Location not consistent with experimental gene/protein characterization data.
 

Comment

  

         Cell line U-2 OS

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Location(s)

Nucleus but not nucleoli Nucleus but not nucleoli 

Staining characteristics

 Granular 

Staining intensity

Moderate Weak 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data.
 
Location not consistent with experimental gene/protein characterization data.
 

Comment

  

         Cell line U-251 MG

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Location(s)

Nucleus but not nucleoli, Golgi apparatus Nucleus but not nucleoli 

Staining characteristics

  

Staining intensity

Weak Moderate 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data.
 
Location not consistent with experimental gene/protein characterization data.
 

Comment

  

         Antibody summary


Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleus but not nucleoli.
 

Immunofluorescent staining of human cell line U-251 MG shows positivity in nucleus but not nucleoli.
 

Summary

Staining of nucleus but not nucleoli in all three cell lines with additional staining of Golgi in U-251 MG.   Staining of the nucleus in all three cell lines.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is not consistent with literature.
 

Dictionary

Microtubules
Golgi apparatus
Nucleus but not nucleoli