Fields »

SUBCELLULAR LOCATION

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Main location(s)

Nucleus but not nucleoli

Additional location(s)

Cytoplasm 

Staining summary

Mainly localized to the nucleus.

Reliability (APE)

Medium

Antibodies in assay

HPA030413, HPA030414, HPA030415
 

Antibody HPA030413

 

Antibody HPA030414

 

Antibody HPA030415

 

         Cell line A-431

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Location(s)

Nucleus but not nucleoli Nucleus but not nucleoli  

Staining characteristics

   

Staining intensity

Strong Weak Negative 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but supported by ≥1 other antibody.
 
One/multiple location(s) with no available experimental gene/protein characterization data.
 
No staining.
 

Comment

   

         Cell line U-2 OS

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Location(s)

Nucleus but not nucleoli   

Staining characteristics

   

Staining intensity

Weak Negative Negative 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but supported by ≥1 other antibody.
 
No staining.
 
No staining.
 

Comment

   

         Cell line U-251 MG

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Location(s)

Nucleus but not nucleoli Cytoplasm Nucleus but not nucleoli 

Staining characteristics

   

Staining intensity

Weak Weak Weak 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but supported by ≥1 other antibody.
 
One/multiple location(s) with no available experimental gene/protein characterization data.
 
One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but supported by ≥1 other antibody.
 

Comment

   

         Antibody summary


Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleus but not nucleoli.
 

Immunofluorescent staining of human cell line A-431 shows positivity in nucleus but not nucleoli.
 

Immunofluorescent staining of human cell line U-251 MG shows positivity in nucleus but not nucleoli.
 

Summary

Staining of nucleus in all three cell lines.   Staining of the cytoplasm in U-251 MG and the nucleus in A-431.   Staining of the nucleus in U-251 MG.  

Validation

The subcellular location is supported by literature.
 
The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is supported by literature.
 

Dictionary

Cytoplasm
Nucleus but not nucleoli