CMTM1

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA013801

 

Antibody HPA017136

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 Atlas Antibodies
Sigma-Aldrich
 

Product name

HPA013801 HPA017136 

Host species

Rabbit Rabbit 

Clonality

pAb pAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand Affinity purified using the PrEST-antigen as affinity ligand 

Released in version

4 5 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment Recombinant protein fragment 

Length (aa)

33 26 

Antigen sequence

DPEHAKPESSEAPSGNLKQPETAAALASSGSVD
 
TKMRTNLKRFLGVEVERKLSPAKDAY
 

Matching transcripts

CMTM1-002 - ENSP00000331428 [100%]
CMTM1-001 - ENSP00000368814 [97%]
CMTM1-006 - ENSP00000435786 [97%]
CMTM1-026 - ENSP00000330061 [97%]
CMTM1-005 - ENSP00000337119 [82%]
CMTM1-013 - ENSP00000432687 [82%]
CMTM1-018 - ENSP00000432387 [82%]
 CMTM1-001 - ENSP00000368814 [100%]
CMTM1-002 - ENSP00000331428 [100%]
CMTM1-005 - ENSP00000337119 [100%]
CMTM1-006 - ENSP00000435786 [100%]
CMTM1-015 - ENSP00000405729 [100%]
CMTM1-017 - ENSP00000434434 [100%]
 

Other gene match

 CKLF-CMTM1 - ENSG00000254788 [100%]
 

ANTIBODY VALIDATION

         Immunohistochemistry

Image

  

Description

Immunohistochemical staining of human prostate shows moderate membranous positivity in glandular cells.
More information
 Immunohistochemical staining of human prostate shows strong cytoplasmic positivity in glandular cells.
More information
 

Retrieval method

HIER pH6 HIER pH6 

Antibody dilution

1:20 1:35 

Literature conformity

No avaliable characterization data No avaliable characterization data 

RNA consistency

Not done Not done 

         Immunofluorescence

Image

  

Description

Immunofluorescent staining of human cell line U-251 MG shows positivity in nucleus but excluded from the nucleoli.
More information
 Application not done for this antibody. 

Antibody dilution

1:43  

Validation IF

Uncertain: The subcellular location is partly supported by literature or no literature is available.  

         Western Blot

Image

  

Description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 

Target mass (kDa)

31.1, 25.5, 25.1, 13.0, 12.5, 6.6, 3.4 31.1, 25.5, 18.6, 13.0, 12.9, 12.5, 7.8 

Antibody dilution

1:250 1:250 

Validation WB

Non-supportive: Only bands not corresponding to the predicted size Uncertain: Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data 

         Protein array

Image

  

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 

Antibody dilution

1:3000 1:3000 

Validation PA

Supportive: Pass with single peak corresponding to interaction only with its own antigen. Supportive: Pass with single peak corresponding to interaction only with its own antigen. 
 

ANTIGEN VIEW

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CMTM1-001
 
CMTM1-002
 
CMTM1-005
 
CMTM1-006
 
CMTM1-013
 
CMTM1-015
 
CMTM1-017
 
CMTM1-018
 
CMTM1-026