The normal tissue page shows antibody staining and, when available, knowledge-based annotated protein expression in 46 human tissues. The assay and annotation is described here.
This page starts with a summary. The summary data can be based on one or more antibodies (reported by the "Antibodies in assay"-field). If the data is based on one single antibody, the IH validation score for that antibody is reported in the "Reliability (Single)"-field (color-coding described here). If the data is based on more than one antibody, the reliability score for the data is reported as "Reliability (APE)" (color-coding described here).
The tissues and cell types can be ordered by organ, cell type or alphabetically.
For each tissue and cell type, the antibody staining is given with the yellow-scale color-coding (described by the scale in the box to the right). Each available antibody is listed in a separate column and the antibody identifier is available at the bottom of the tissue list. The images and annotations can be accessed by clicking on the tissue name.
When available, a knowledge-based annotated protein expression (APE) is given to the right of the antibody staining values. The level of expression is given by a blue-scale color-coding (described by the scale in the box to the right).
At the very bottom of this page, a summary of the staining from each antibody is given, together with a representative image and the immunohistochemistry validation score for the antibody.
Cytoplasmic expression in a subset of tissues including prostate glands, cells in intestine, gall bladder and renal tubuli.
Caution: Based on antibodies targeting proteins from multiple genes.
Immunohistochemical staining of human prostate shows moderate membranous positivity in glandular cells.
Immunohistochemical staining of human prostate shows strong cytoplasmic positivity in glandular cells.
Summary
A majority of normal tissues displayed weak to moderate cytoplasmic and membranous positivity. Hepatocytes, glandular cells in gall bladder and upper layers in most squamous epithelia showed strong staining. Myocytes and lymphoid cells were in general negative.
Most normal tissues were weakly stained or negative. Subtypes of lymphoid, bone marrow poietic and prostate gland cells displayed strong immunoreactivity. Cells in the intestine, gall bladder and renal tubuli exhibited moderate staining in cytoplasm and luminal membranes.
Validation
One antibody yielding a staining pattern and there is no available gene/protein characterization data or the staining pattern is partly consistent with or contradicted by limited gene/protein characterization data
The multi-targeting antibody yielding a staining pattern partly consistent with limited gene/protein characterization data or there is no available gene/protein characterization data for all or at least one of the genes
