Fields »

SUBCELLULAR LOCATION

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Main location(s)

Nucleus but not nucleoli

Additional location(s)

 

Staining summary

Localized to the nucleus.

Reliability (APE)

Very low

Antibodies in assay

HPA013801, HPA017136
 

Antibody HPA013801

 

Antibody HPA017136

 

         Cell line A-431

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Location(s)

Nucleus but not nucleoli Nucleoli 

Staining characteristics

  

Staining intensity

Weak Weak 

Validation

Location not consistent with experimental gene/protein characterization data and showing dissimilar staining to ≥1 other antibody.
 
The multi-targeting antibody yielding a staining pattern with no available gene/protein characterization data.
 

Comment

  

         Cell line U-2 OS

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Location(s)

Nucleus but not nucleoli  

Staining characteristics

  

Staining intensity

Weak Negative 

Validation

Location not consistent with experimental gene/protein characterization data and showing dissimilar staining to ≥1 other antibody.
 
The multi-targeting antibody not yielding a staining pattern.
 

Comment

  

         Cell line U-251 MG

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Location(s)

Nucleus but not nucleoli   

Staining characteristics

   

Staining intensity

Weak   

Validation

Location not consistent with experimental gene/protein characterization data and showing dissimilar staining to ≥1 other antibody.
   

Comment

   

         Antibody summary


Immunofluorescent staining of human cell line U-251MG shows positivity in nuclei but not nucleoli.
 

Immunofluorescent staining of human cell line A-431 shows positivity in nucleoli.
 

Summary

Staining of nuclei but not nucleoli in all three cell lines.   Staining of nucleoli in A-431.  

Validation

The subcellular location is not consistent with literature.
 
The subcellular location is partly supported by literature or no literature is available.
 

Dictionary

Nucleus but not nucleoli