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SUBCELLULAR LOCATION

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Main location(s)

Nucleus but not nucleoli, Vesicles

Additional location(s)

Golgi apparatus 

Staining summary

Mainly localized to the nucleus and vesicles but also to the Golgi apparatus.

Reliability (APE)

Medium

Antibodies in assay

HPA015305, HPA017869
 

Antibody HPA015305

 

Antibody HPA017869

 

         Cell line A-431

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Location(s)

Nucleus but not nucleoli, Golgi apparatus, Vesicles Nucleus but not nucleoli, Vesicles 

Staining characteristics

  

Staining intensity

Moderate Moderate 

Validation

Not decisive - One/multiple location(s) where experimental gene/protein characterization data is partly supporting and partly conflicting.
 
Not decisive - One/multiple location(s) where experimental gene/protein characterization data is partly supporting and partly conflicting.
 

Comment

  

         Cell line U-2 OS

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Location(s)

Nucleus but not nucleoli, Golgi apparatus, Vesicles Nucleus but not nucleoli, Vesicles 

Staining characteristics

  

Staining intensity

Moderate Moderate 

Validation

Not decisive - One/multiple location(s) where experimental gene/protein characterization data is partly supporting and partly conflicting.
 
Not decisive - One/multiple location(s) where experimental gene/protein characterization data is partly supporting and partly conflicting.
 

Comment

  

         Cell line U-251 MG

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Location(s)

Nucleus but not nucleoli, Golgi apparatus, Vesicles Nucleus but not nucleoli 

Staining characteristics

  

Staining intensity

Moderate Weak 

Validation

Not decisive - One/multiple location(s) where experimental gene/protein characterization data is partly supporting and partly conflicting.
 
Location not consistent with experimental gene/protein characterization data.
 

Comment

  

         Antibody summary


Immunofluorescent staining of human cell line U-251MG shows positivity in nucleus but not nucleoli, golgi apparatus & vesicles.
 

Immunofluorescent staining of human cell line U-251MG shows positivity in nucleus but not nucleoli.
 

Summary

Staining of Golgi, vesicles and nucleus in all three cell lines.   Staining of nucleus but not nucleoli in all three cell lines with additional staining of vesicles in A-431 and U-2 OS.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is partly supported by literature or no literature is available.
 

Dictionary

Golgi apparatus
Nucleus but not nucleoli
Endosome