The normal tissue page shows antibody staining and, when available, knowledge-based annotated protein expression in 46 human tissues. The assay and annotation is described here.
This page starts with a summary. The summary data can be based on one or more antibodies (reported by the "Antibodies in assay"-field). If the data is based on one single antibody, the IH validation score for that antibody is reported in the "Reliability (Single)"-field (color-coding described here). If the data is based on more than one antibody, the reliability score for the data is reported as "Reliability (APE)" (color-coding described here).
The tissues and cell types can be ordered by organ, cell type or alphabetically.
For each tissue and cell type, the antibody staining is given with the yellow-scale color-coding (described by the scale in the box to the right). Each available antibody is listed in a separate column and the antibody identifier is available at the bottom of the tissue list. The images and annotations can be accessed by clicking on the tissue name.
When available, a knowledge-based annotated protein expression (APE) is given to the right of the antibody staining values. The level of expression is given by a blue-scale color-coding (described by the scale in the box to the right).
At the very bottom of this page, a summary of the staining from each antibody is given, together with a representative image and the immunohistochemistry validation score for the antibody.
Immunohistochemical staining of human adrenal gland shows strong granular cytoplasmic positivity in cortical cells.
Immunohistochemical staining of human rectum shows moderate cytoplasmic and membranous positivity in glandular cells.
Immunohistochemical staining of human cerebellum shows strong nuclear positivity in Purkinje cells.
Summary
A majority of normal tissues displayed moderate cytoplasmic positivity. Islets cells in pancreas and cortical cells in adrenal gland were strongly stained. cells in CNS and lymphoid tissues were generally negative.
Malignancies showed weak to moderate cytoplasmic positivity. Membranous staining was also found. Most gliomas and renal carcinomas were negative.
Most of the normal tissues displayed weak to moderate cytoplasmic and membranous positivity. Neuronal cells of CNS showed nuclear/nucleolar staining. Hepatocytes, glial cells and lymphoid tissues were negative.
A majority of normal cells showed weak to moderate nuclear and cytoplasmic positivity combined with a few cases of membranous staining. Strong membranous positivity was observed in urothelium while neuronal cells displayed strong nuclear immunoreactivity. Lung and lymphoid tissues were generally negative.
Validation
Two (or more) antibodies yielding similar staining patterns which are consistent with available gene/protein characterization data
Two (or more) antibodies yielding similar staining patterns which are consistent with available gene/protein characterization data
Two (or more) antibodies yielding partly similar staining patterns which are partly consistent with gene/protein characterization data or consistent with limited gene/protein characterization data
