Fields »

SUBCELLULAR LOCATION

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Main location(s)

Nucleus, Cytoplasm

Additional location(s)

 

Staining summary

Localized to nucleus and cytoplasm.

Reliability (APE)

Very low

Antibodies in assay

HPA001654, HPA003732, CAB022214
 

Antibody HPA001654

 

Antibody HPA003732

 

Antibody CAB022214

 

         Cell line A-431

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Location(s)

Nucleus Cytoplasm  

Staining characteristics

Granular Granular  

Staining intensity

Weak Moderate Negative 

Validation

Not decisive - One/multiple location(s) where experimental gene/protein characterization data is partly supporting and partly conflicting.
 
Not decisive - One/multiple location(s) where experimental gene/protein characterization data is partly supporting and partly conflicting.
 
No staining.
 

Comment

   

         Cell line U-2 OS

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Location(s)

Nucleus Cytoplasm  

Staining characteristics

Granular   

Staining intensity

Weak Moderate Negative 

Validation

Not decisive - One/multiple location(s) where experimental gene/protein characterization data is partly supporting and partly conflicting.
 
Not decisive - One/multiple location(s) where experimental gene/protein characterization data is partly supporting and partly conflicting.
 
No staining.
 

Comment

   

         Cell line U-251 MG

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Location(s)

Nucleus Nucleus but not nucleoli, Cytoplasm  

Staining characteristics

Granular Granular  

Staining intensity

Weak Moderate Negative 

Validation

Not decisive - One/multiple location(s) where experimental gene/protein characterization data is partly supporting and partly conflicting.
 
Not decisive - One/multiple location(s) where experimental gene/protein characterization data is partly supporting and partly conflicting.
 
No staining.
 

Comment

   

         Antibody summary


Immunofluorescent staining of human cell line U-251MG shows positivity in nuclei.
 

Immunofluorescent staining of human cell line A-431 shows positivity in cytoplasm.
 

Immunofluorescent staining of human cell line A-431 shows no positivity.
 

Summary

Granular staining of nuclei and cytoplasm in all three cell lines.   Staining of cytoplasm in all three cell lines. Additional staining of nucleus in U-251MG.   No staining was observed in any of the three cell lines.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is partly supported by literature or no literature is available.
 

Dictionary

Cytoplasm
Nucleus