Fields »

SUBCELLULAR LOCATION

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Main location(s)

Nucleus but not nucleoli

Additional location(s)

 

Staining summary

Localized to the nucleus.

Reliability (APE)

High

Antibodies in assay

HPA002928, CAB005113
 

Antibody HPA002928

 

Antibody CAB005113

 

         Cell line A-431

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Location(s)

Nucleus but not nucleoli Nucleus but not nucleoli 

Staining characteristics

Granular  

Staining intensity

Moderate Moderate 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data.
 
One/multiple location(s) supported by experimental gene/protein characterization data.
 

Comment

  

         Cell line U-2 OS

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Location(s)

Nucleus but not nucleoli Nucleus but not nucleoli 

Staining characteristics

  

Staining intensity

Weak Weak 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data.
 
One/multiple location(s) supported by experimental gene/protein characterization data.
 

Comment

  

         Cell line U-251 MG

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Location(s)

Nucleus but not nucleoli Nucleus but not nucleoli, Cytoplasm 

Staining characteristics

Granular  

Staining intensity

Moderate Weak 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data.
 
Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 

Comment

  

         Antibody summary


Immunofluorescent staining of human cell line U-251MG shows positivity in nuclei but not nucleoli.
 

Immunofluorescent staining of human cell line A-431 shows positivity in nucleus but not nucleoli.
 

Summary

Staining of nuclei but not nucleoli in all three cell lines.   Staining of nucleus in all three cell lines, with additional staining of cytoplasm in U-251 MG.  

Validation

The subcellular location is supported by literature.
 
The subcellular location is supported by literature.
 

Dictionary

Nucleus but not nucleoli