Fields »

SUBCELLULAR LOCATION

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Main location(s)

Plasma membrane

Additional location(s)

Cytoplasm 

Staining summary

Mainly localized to the plasma membrane. Additionally localized to the cytoplasm.

Reliability (APE)

High

Antibodies in assay

CAB004035, HPA021616, CAB047324
 

Antibody CAB004035

 

Antibody HPA021616

 

Antibody CAB047324

 

         Cell line A-431

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Location(s)

Plasma membrane, Cytoplasm Plasma membrane Plasma membrane 

Staining characteristics

   

Staining intensity

Moderate Strong Moderate 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data.
 
One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 
One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 

Comment

   

         Cell line U-2 OS

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Location(s)

Plasma membrane Nucleoli, Plasma membrane Plasma membrane 

Staining characteristics

 Cluster  

Staining intensity

Moderate Strong Weak 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data.
 
One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 
One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 

Comment

   

         Cell line U-251 MG

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Location(s)

Plasma membrane, Cytoplasm Plasma membrane Plasma membrane 

Staining characteristics

   

Staining intensity

Moderate Strong Weak 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data.
 
One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 
One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 

Comment

   

         Antibody summary


Immunofluorescent staining of human cell line A-431 shows positivity in plasma membrane & cytoplasm.
 

Immunofluorescent staining of human cell line A-431 shows positivity in plasma membrane.
 

Immunofluorescent staining of human cell line U-2 OS shows positivity in plasma membrane.
 

Summary

Staining of plasma membrane in all three cell lines with additional staining of cytoplasm in A-431 and U-251 MG.   Staining of plasma membrane in all three cell lines with additional staining of nucleoli in U-2 OS.   Staining of plasma membrane in all three cell lines.  

Validation

The subcellular location is supported by literature.
 
The subcellular location is supported by literature.
 
The subcellular location is supported by literature.
 

Dictionary

Cytoplasm
Plasma membrane