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SUBCELLULAR LOCATION

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Main location(s)

Nuclear membrane

Additional location(s)

Nucleus but not nucleoli, Cytoplasm, Centrosome 

Staining summary

Staining of nuclear membrane in all three cell lines with additional staining of nucleus and cytoplasm in U-251 MG and centrosome in A-431.

Reliability (Single)

Antibodies in assay

HPA040086
 

Antibody HPA040086

 

         Cell line A-431

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Location(s)

Nuclear membrane, Centrosome 

Staining characteristics

 

Staining intensity

Weak 

Validation

Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 

Comment

 

         Cell line U-2 OS

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Location(s)

Nuclear membrane 

Staining characteristics

 

Staining intensity

Weak 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data.
 

Comment

 

         Cell line U-251 MG

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Location(s)

Nucleus but not nucleoli, Nuclear membrane, Cytoplasm 

Staining characteristics

 

Staining intensity

Weak 

Validation

Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 

Comment

 

         Antibody summary


Immunofluorescent staining of human cell line U-2 OS shows positivity in nuclear membrane.
 

Summary

Staining of nuclear membrane in all three cell lines with additional staining of nucleus and cytoplasm in U-251 MG and centrosome in A-431.  

Validation

The subcellular location is supported by literature.
 

Dictionary

Centrosome
Cytoplasm
Nuclear membrane
Nucleus but not nucleoli