The normal tissue page shows antibody staining and, when available, knowledge-based annotated protein expression in 46 human tissues. The assay and annotation is described here.
This page starts with a summary. The summary data can be based on one or more antibodies (reported by the "Antibodies in assay"-field). If the data is based on one single antibody, the IH validation score for that antibody is reported in the "Reliability (Single)"-field (color-coding described here). If the data is based on more than one antibody, the reliability score for the data is reported as "Reliability (APE)" (color-coding described here).
The tissues and cell types can be ordered by organ, cell type or alphabetically.
For each tissue and cell type, the antibody staining is given with the yellow-scale color-coding (described by the scale in the box to the right). Each available antibody is listed in a separate column and the antibody identifier is available at the bottom of the tissue list. The images and annotations can be accessed by clicking on the tissue name.
When available, a knowledge-based annotated protein expression (APE) is given to the right of the antibody staining values. The level of expression is given by a blue-scale color-coding (described by the scale in the box to the right).
At the very bottom of this page, a summary of the staining from each antibody is given, together with a representative image and the immunohistochemistry validation score for the antibody.
Immunohistochemical staining of human duodenum shows moderate cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human heart muscle shows strong cytoplasmic positivity in myocytes.
Immunohistochemical staining of human prostate shows cytoplasmic and membranous positivity in glandular cells.
Immunohistochemical staining of human adrenal gland shows strong nuclear positivity in cortical cells.
Summary
A subset of cells in lymphoid tissues showed strong cytoplasmic positivity. Most remaining normal tissues were weakly stained or negative.
Normal tissues showed moderate cytoplasmic and nuclear staining. Intestinal tract, myocytes and exocrine pancreas were strongly stained. Cilia of fallopian tube showed strong positivity. Bile duct cells, islet cells and most cells in CNS were weakly stained or negative.
Most normal tissues displayed moderate cytoplasmic and/or nuclear positivity. Strong membranous staining was observed in gall bladder, throphoblast cells and in a subset of cells in the intestinal tract. Most cells in CNS and lymphoid tissues were weakly stained or negative.
Most normal tissues displayed weak to moderate cytoplasmic and nuclear immunoreactivity. Cortical cells in adrenal gland showed strong nuclear positivity. CNS tissue was weakly stained.
Validation
Two (or more) antibodies yielding partly similar staining patterns which are partly consistent with gene/protein characterization data or consistent with limited gene/protein characterization data
Two (or more) antibodies yielding partly similar staining patterns which are partly consistent with gene/protein characterization data or consistent with limited gene/protein characterization data
Two (or more) antibodies yielding partly similar staining patterns which are partly consistent with gene/protein characterization data or consistent with limited gene/protein characterization data
Two (or more) antibodies yielding partly similar staining patterns which are partly consistent with gene/protein characterization data or consistent with limited gene/protein characterization data
