Fields »

SUBCELLULAR LOCATION

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Main location(s)

Nucleus, Nucleus but not nucleoli

Additional location(s)

Nucleoli, Cytoplasm 

Staining summary

Localized to the nucleus but excluded from the nucleolus.

Reliability (APE)

High

Antibodies in assay

HPA027280, HPA028409
 

Antibody HPA027280

 

Antibody HPA028409

 

         Cell line A-431

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Location(s)

Nucleus but not nucleoli Nucleus but not nucleoli 

Staining characteristics

Spotty  

Staining intensity

Strong Weak 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 
One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 

Comment

  

         Cell line U-2 OS

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Location(s)

Nucleus but not nucleoli Nucleus but not nucleoli 

Staining characteristics

  

Staining intensity

Moderate Weak 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 
One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 

Comment

  

         Cell line U-251 MG

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Location(s)

Nucleus but not nucleoli Nucleus but not nucleoli 

Staining characteristics

Spotty  

Staining intensity

Strong Weak 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 
One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 

Comment

  

         Antibody summary


Immunofluorescent staining of human cell line U-251MG shows positivity in nuclei but not nucleoli.
 

Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleus but not nucleoli.
 

Summary

Staining of nuclei but not nucleoli in all three cell lines.   Staining of nucleus in all three cell lines.  

Validation

The subcellular location is supported by literature.
 
The subcellular location is supported by literature.
 

Dictionary

Cytoplasm
Nucleoli
Nucleus
Nucleus but not nucleoli