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SUBCELLULAR LOCATION

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Main location(s)

Nucleus but not nucleoli

Additional location(s)

Cytoskeleton (Microtubules) 

Staining summary

Mainly localized to the nucleus. In addition localized to the microtubules. Caution: Based on antibodies targeting proteins from multiple genes.

Reliability (APE)

High

Antibodies in assay

HPA003995, CAB004229, HPA030069
 

Antibody HPA003995

 

Antibody CAB004229

 

Antibody HPA030069

 

         Cell line A-431

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Location(s)

Nucleus but not nucleoli, Cytoskeleton (Microtubules)  Nucleus but not nucleoli 

Staining characteristics

   

Staining intensity

Moderate Negative Weak 

Validation

The multi-targeting antibody yielding a staining pattern consistent with available gene/protein characterization data for all of the genes.
 
No staining.
 
The multi-targeting antibody yielding a staining pattern consistent with available gene/protein characterization data for all of the genes.
 

Comment

   

         Cell line U-2 OS

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Location(s)

Nucleus but not nucleoli, Cytoskeleton (Microtubules)    

Staining characteristics

    

Staining intensity

Moderate Negative   

Validation

The multi-targeting antibody yielding a staining pattern consistent with available gene/protein characterization data for all of the genes.
 
No staining.
   

Comment

    

         Cell line U-251 MG

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Location(s)

Nucleus but not nucleoli, Cytoskeleton (Microtubules)  Nucleus but not nucleoli 

Staining characteristics

  Granular 

Staining intensity

Moderate Negative Weak 

Validation

The multi-targeting antibody yielding a staining pattern consistent with available gene/protein characterization data for all of the genes.
 
No staining.
 
The multi-targeting antibody yielding a staining pattern consistent with available gene/protein characterization data for all of the genes.
 

Comment

   

         Antibody summary


Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleus but not nucleoli & cytoskeleton (microtubules).
 

Immunofluorescent staining of human cell line U-2 OS shows no positivity.
 

Immunofluorescent staining of human cell line U-251 MG shows positivity in nucleus but not nucleoli.
 

Summary

Staining of cytoskeleton (microtubules) and nucleus in all three cell lines.   No staining was observed in any of the three cell lines.   Staining of nucleus in all three cell lines.  

Validation

The subcellular location is supported by literature.
 
The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is supported by literature.
 

Dictionary

Microtubules
Nucleus but not nucleoli