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SUBCELLULAR LOCATION

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Main location(s)

Plasma membrane, Cytoplasm

Additional location(s)

Cytoskeleton (Actin filaments) 

Staining summary

Staining of plasma membrane and cytoplasm in all three cell lines with additional staining of Actin filaments in U-2 OS.

Reliability (Single)

Antibodies in assay

HPA000845
 

Antibody HPA000845

 

         Cell line A-431

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Location(s)

Plasma membrane, Cytoplasm 

Staining characteristics

 

Staining intensity

Strong 

Validation

Location not consistent with experimental gene/protein characterization data.
 

Comment

 

         Cell line U-2 OS

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Location(s)

Plasma membrane, Cytoplasm, Cytoskeleton (Actin filaments) 

Staining characteristics

Fibrous 

Staining intensity

Moderate 

Validation

Location not consistent with experimental gene/protein characterization data.
 

Comment

 

         Cell line U-251 MG

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Location(s)

Plasma membrane, Cytoplasm 

Staining characteristics

 

Staining intensity

Moderate 

Validation

Location not consistent with experimental gene/protein characterization data.
 

Comment

 

         Antibody summary


Immunofluorescent staining of human cell line U-2 OS shows positivity in plasma membrane, cytoplasm & cytoskeleton (actin filaments).
 

Summary

Staining of plasma membrane and cytoplasm in all three cell lines with additional staining of Actin filaments in U-2 OS.  

Validation

The subcellular location is not consistent with literature.
 

Dictionary

Cytoplasm
Actin filaments
Plasma membrane