MYH9

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA001644

 

Antibody CAB015386

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 Santa Cruz Biotechnology
 

Product name

HPA001644 sc-53095 

Host species

Rabbit Mouse 

Clonality

pAb mAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand Protein A/G 

Released in version

3 4 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment Not known 

Length (aa)

139  

Antigen sequence

REQEVNILKKTLEEEAKTHEAQIQEMRQKHSQAVEELAEQLEQTKRVKAN
LEKAKQTLENERGELANEVKVLLQGKGDSEHKRKKVEAQLQELQVKFNEG
ERVRTELADKVTKLQVELDNVTGLLSQSDSKSSKLTKDF
 
 

Matching transcripts

MYH9-001 - ENSP00000216181 [100%]
  

Other gene match

  

ANTIBODY VALIDATION

         Immunohistochemistry

Image

  

Description

Immunohistochemical staining of human kidney shows distinct cytoplasmic positivity in cells in glomeruli.
More information
 Immunohistochemical staining of human kidney shows distinct cytoplasmic positivity in cells in glomeruli.
More information
 

Retrieval method

HIER pH6 HIER pH6 

Antibody dilution

1:1000 1:30 

Validation IH

Uncertain: Two (or more) antibodies yielding partly similar staining patterns which are partly consistent with gene/protein characterization data or consistent with limited gene/protein characterization data Uncertain: Two (or more) antibodies yielding partly similar staining patterns which are partly consistent with gene/protein characterization data or consistent with limited gene/protein characterization data 

         Immunofluorescence

Image

  

Description

Immunofluorescent staining of human cell line U-251 MG shows positivity in plasma membrane, cytoplasm & actin filaments.
More information
 Immunofluorescent staining of human cell line A-431 shows positivity in cell junctions.
More information
 

Antibody dilution

1:20 1:8 

Validation IF

Uncertain: The subcellular location is partly supported by literature or no literature is available. Uncertain: The subcellular location is partly supported by literature or no literature is available. 

         Western Blot

Image

  

Description

Target too small/large to be analyzed with the present setup Lane 1: Marker [kDa] 229, 107, 78, 46, 31, 26, 16.2
Lane 2: RT-4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 

Target mass (kDa)

226.5 226.5, 24.8, 11.6 

Antibody dilution

 1:500 

Validation WB

 Uncertain: No bands detected 

         Protein array

Image

  

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
  

Antibody dilution

1:3000  

Validation PA

Uncertain: Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).  
 

ANTIGEN VIEW

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MYH9-001