Fields »

SUBCELLULAR LOCATION

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Main location(s)

Nucleus but not nucleoli, Cytoplasm

Additional location(s)

 

Staining summary

Localized to nucleus and cytoplasm.

Reliability (APE)

High

Antibodies in assay

HPA005788, CAB022073
 

Antibody HPA005788

 

Antibody CAB022073

 

         Cell line A-431

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Location(s)

Nucleus but not nucleoli   

Staining characteristics

Granular   

Staining intensity

Moderate   

Validation

One/multiple location(s) supported by experimental gene/protein characterization data.
   

Comment

   

         Cell line U-2 OS

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Location(s)

Cytoplasm Cytoplasm 

Staining characteristics

Granular, Fibrous  

Staining intensity

Moderate Weak 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data.
 
One/multiple location(s) supported by experimental gene/protein characterization data.
 

Comment

  

         Cell line U-251 MG

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Location(s)

Nucleus but not nucleoli, Cytoplasm Cytoplasm 

Staining characteristics

Granular  

Staining intensity

Moderate Weak 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data.
 
One/multiple location(s) supported by experimental gene/protein characterization data.
 

Comment

  

         Antibody summary


Immunofluorescent staining of human cell line U-251MG shows positivity in nucleus but not nucleoli & cytoplasm.
 

Immunofluorescent staining of human cell line U-2 OS shows positivity in cytoplasm.
 

Summary

Staining of nucleus in A-431 and cytoplasm in U-2 OS. Staining of both cytoplasm and nucleus in U-251 MG.   Staining of cytoplasm in U-251 MG and U-2 OS.  

Validation

The subcellular location is supported by literature.
 
The subcellular location is supported by literature.
 

Dictionary

Cytoplasm
Nucleus but not nucleoli