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SUBCELLULAR LOCATION

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Main location(s)

Nucleus but not nucleoli

Additional location(s)

Cytoplasm, Centrosome 

Staining summary

Mainly localized to the nucleus but excluded from the nucleoli.

Reliability (APE)

High

Antibodies in assay

CAB000146, HPA003128, HPA004112
 

Antibody CAB000146

 

Antibody HPA003128

 

Antibody HPA004112

 

         Cell line A-431

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Location(s)

 Nucleus but not nucleoli, Cytoplasm Nucleus but not nucleoli 

Staining characteristics

 Granular  

Staining intensity

Negative Strong Strong 

Validation

No staining.
 
One/multiple location(s) supported by experimental gene/protein characterization data.
 
One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 

Comment

   

         Cell line U-2 OS

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Location(s)

Nucleus but not nucleoli Nucleus but not nucleoli, Cytoplasm Nucleus but not nucleoli 

Staining characteristics

 Smooth  

Staining intensity

Weak Strong Strong 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 
One/multiple location(s) supported by experimental gene/protein characterization data.
 
One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 

Comment

   

         Cell line U-251 MG

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Location(s)

Nucleus but not nucleoli Nucleus but not nucleoli, Cytoplasm Nucleus but not nucleoli 

Staining characteristics

 Granular  

Staining intensity

Weak Moderate Strong 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 
One/multiple location(s) supported by experimental gene/protein characterization data.
 
One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 

Comment

   

         Antibody summary


Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleus but not nucleoli.
 

Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleus but not nucleoli & cytoplasm.
 

Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleus but not nucleoli.
 

Summary

Staining of nucleus in U-251 MG and U-2 OS.   Staining of nucleus and cytoplasm in all three cell lines.   Staining of nucleus in all three cell lines.  

Validation

The subcellular location is supported by literature.
 
The subcellular location is supported by literature.
 
The subcellular location is supported by literature.
 

Dictionary

Centrosome
Cytoplasm
Nucleus but not nucleoli