The normal tissue page shows antibody staining and, when available, knowledge-based annotated protein expression in 46 human tissues. The assay and annotation is described here.
This page starts with a summary. The summary data can be based on one or more antibodies (reported by the "Antibodies in assay"-field). If the data is based on one single antibody, the IH validation score for that antibody is reported in the "Reliability (Single)"-field (color-coding described here). If the data is based on more than one antibody, the reliability score for the data is reported as "Reliability (APE)" (color-coding described here).
The tissues and cell types can be ordered by organ, cell type or alphabetically.
For each tissue and cell type, the antibody staining is given with the yellow-scale color-coding (described by the scale in the box to the right). Each available antibody is listed in a separate column and the antibody identifier is available at the bottom of the tissue list. The images and annotations can be accessed by clicking on the tissue name.
When available, a knowledge-based annotated protein expression (APE) is given to the right of the antibody staining values. The level of expression is given by a blue-scale color-coding (described by the scale in the box to the right).
At the very bottom of this page, a summary of the staining from each antibody is given, together with a representative image and the immunohistochemistry validation score for the antibody.
Strong cytoplasmic staining was observed in hepatocytes, plasma cells, parietal cells of stomach and in islet cells of pancreas. Hepatocytes showed an additional nuclear positivity. Other epithelia were generally moderately stained in cytoplasm, often of granular character and accentuated in basal or luminal position. In some cases the positivity was combined with weakly stained nuclei. In lymphoid cells outside reaction centra, nuclear staining accentuated to the nuclear membrane was observed. Positivity was also found inside blood vessels and other extra-cellular lumina.
Immunohistochemical staining of human liver shows strong cytoplasmic and nuclear positivity in hepatocytes.
Summary
Strong cytoplasmic staining was observed in hepatocytes, plasma cells, parietal cells of stomach and in islet cells of pancreas. Hepatocytes showed an additional nuclear positivity. Other epithelia were generally moderately stained in cytoplasm, often of granular character and accentuated in basal or luminal position. In some cases the positivity was combined with weakly stained nuclei. In lymphoid cells outside reaction centra, nuclear staining accentuated to the nuclear membrane was observed. Positivity was also found inside blood vessels and other extra-cellular lumina.
Validation
One antibody yielding a staining pattern which is consistent with gene/protein characterization data
