Fields »

SUBCELLULAR LOCATION

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Main location(s)

Nucleus

Additional location(s)

Cytoplasm 

Staining summary

Mainly localized to the nucleus.

Reliability (APE)

High

Antibodies in assay

HPA000660, HPA017929
 

Antibody HPA000660

 

Antibody HPA017929

 

         Cell line A-431

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Location(s)

Nucleus but not nucleoli, Cytoplasm Nucleus, Nucleoli 

Staining characteristics

Granular  

Staining intensity

Moderate Strong 

Validation

Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 
One/multiple location(s) supported by experimental gene/protein characterization data.
 

Comment

  

         Cell line U-2 OS

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Location(s)

Nucleus but not nucleoli, Cytoplasm Nucleus 

Staining characteristics

Granular, Smooth  

Staining intensity

Strong Moderate 

Validation

Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 
One/multiple location(s) supported by experimental gene/protein characterization data.
 

Comment

  

         Cell line U-251 MG

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Location(s)

Nucleus but not nucleoli Nucleus 

Staining characteristics

Granular Smooth 

Staining intensity

Moderate Weak 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data.
 
One/multiple location(s) supported by experimental gene/protein characterization data.
 

Comment

  

         Antibody summary


Immunofluorescent staining of human cell line U-251MG shows positivity in nucleus but not nucleoli.
 

Immunofluorescent staining of human cell line A-431 shows positivity in nucleus & nucleoli.
 

Summary

Staining of nuclei but not nucleoli in all three cell lines. Additional staining of cytoplasm in A-431 and U-2 OS.   Staining of nucleus in all three cell lines.  

Validation

The subcellular location is supported by literature.
 
The subcellular location is supported by literature.
 

Dictionary

Cytoplasm
Nucleus