Fields »

SUBCELLULAR LOCATION

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Main location(s)

Nucleus

Additional location(s)

 

Staining summary

Localized to the nucleus.

Reliability (APE)

High

Antibodies in assay

HPA002564, CAB004294, CAB047307
 

Antibody HPA002564

 

Antibody CAB004294

 

Antibody CAB047307

 

         Cell line A-431

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Location(s)

Nucleus Nucleus but not nucleoli Nucleus but not nucleoli 

Staining characteristics

Granular Granular  

Staining intensity

Moderate Moderate Moderate 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data.
 
One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 
One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 

Comment

   

         Cell line U-2 OS

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Location(s)

Nucleus Nucleus Nucleus 

Staining characteristics

   

Staining intensity

Moderate Weak Weak 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data.
 
One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 
One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 

Comment

   

         Cell line U-251 MG

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Location(s)

Nucleus, Centrosome Nucleus Nucleus but not nucleoli 

Staining characteristics

Granular   

Staining intensity

Moderate Weak Weak 

Validation

Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 
One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 
One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 

Comment

   

         Antibody summary


Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleus.
 

Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleus.
 

Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleus.
 

Summary

Staining of nucleus in all three cell lines. Additional staining of centrosome in U-251MG.   Staining of nucleus in all three cell lines.   Staining of nucleus in all three cell lines.  

Validation

The subcellular location is supported by literature.
 
The subcellular location is supported by literature.
 
The subcellular location is supported by literature.
 

Dictionary

Nucleus