The normal tissue page shows antibody staining and, when available, knowledge-based annotated protein expression in 46 human tissues. The assay and annotation is described here.
This page starts with a summary. The summary data can be based on one or more antibodies (reported by the "Antibodies in assay"-field). If the data is based on one single antibody, the IH validation score for that antibody is reported in the "Reliability (Single)"-field (color-coding described here). If the data is based on more than one antibody, the reliability score for the data is reported as "Reliability (APE)" (color-coding described here).
The tissues and cell types can be ordered by organ, cell type or alphabetically.
For each tissue and cell type, the antibody staining is given with the yellow-scale color-coding (described by the scale in the box to the right). Each available antibody is listed in a separate column and the antibody identifier is available at the bottom of the tissue list. The images and annotations can be accessed by clicking on the tissue name.
When available, a knowledge-based annotated protein expression (APE) is given to the right of the antibody staining values. The level of expression is given by a blue-scale color-coding (described by the scale in the box to the right).
At the very bottom of this page, a summary of the staining from each antibody is given, together with a representative image and the immunohistochemistry validation score for the antibody.
Immunohistochemical staining of human colon shows strong cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells and decidual cells.
Immunohistochemical staining of human testis shows strong nuclear positivity in primary spermatocytes of seminiferous ducts..
Summary
Most of the normal cells showed moderate to strong cytoplasmic immunoreactivity. Reaction center cells of lymphoid tissues, skeletal muscle cells and glial cells were weakly stained or negative.
Most normal tissues showed weak to moderate cytoplasmic positivity. Alveolar cells, pancreatic islet, renal glomeruli, fallopian tubes, most lymphoid cells and myocytes were in general negative. Placenta displayed strong immunoreactivity.
Most of the normal tissues displayed weak to moderate nuclear and/or cytoplasmic positivity. Additional membranous staining was observed in intestinal tract, kidney, fallopian tubes and endometrial glands. Stomach and adrenal cortex exhibited strong cytoplasmic staining while strong nuclear positivity was observed in primary spermatocytes of seminiferous ducts. Lymphoid tissues and smooth muscle were negative.
Validation
Two (or more) antibodies yielding dissimilar or partly similar staining patterns and there is no available gene/protein characterization data or only limited gene/protein characterization data
Two (or more) antibodies yielding dissimilar or partly similar staining patterns and there is no available gene/protein characterization data or only limited gene/protein characterization data
Two (or more) antibodies yielding dissimilar or partly similar staining patterns and there is no available gene/protein characterization data or only limited gene/protein characterization data
