E2F1

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA008003

 

Antibody CAB000329

 

Antibody CAB019308

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 Santa Cruz Biotechnology
 SDIX
 

Product name

HPA008003 sc-251 2851.00.02 

Host species

Rabbit Mouse Rabbit 

Clonality

pAb mAb msAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand Protein A/G Affinity 

Released in version

5 4 5 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment Recombinant protein Genetic immunization 

Length (aa)

127   

Antigen sequence

ISPGKTPSQEVTSEEENRATDSATIVSPPPSSPPSSLTTDPSQSLLSLEQ
EPLLSRMGSLRAPVDEDRLSPLVAADSLLEHVREDFSGLLPEEFISLSPP
HEALDYHFGLEEGEGIRDLFDCDFGDL
 
 
 

Matching transcripts

E2F1-001 - ENSP00000345571 [100%]
   

Other gene match

   

ANTIBODY VALIDATION

         Immunohistochemistry

Image

   

Description

Immunohistochemical staining of human rectum shows strong nuclear positivity in glandular cells.
More information
 Immunohistochemical staining of human testis shows strong nuclear positivity in cells of seminiferus ducts.
More information
 Immunohistochemical staining of human colon shows strong nuclear positivity in glandular cells.
More information
 

Retrieval method

HIER pH6 HIER pH6 HIER pH6 

Antibody dilution

1:100 1:75 1:250 

Validation IH

Supportive: One antibody yielding a staining pattern which is consistent with gene/protein characterization data Supportive: Two (or more) antibodies yielding partly similar staining patterns which are consistent with gene/protein characterization data Supportive: Two (or more) antibodies yielding partly similar staining patterns which are consistent with gene/protein characterization data 

         Western Blot

Image

   

Description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: Negative control (vector only transfected HEK293T lysate)
Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY417432)
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Target mass (kDa)

46.9 46.9 46.9 

Antibody dilution

1:250 1:500 1:500 

Validation WB

Supportive: Band of predicted size in kDa (+/-20%) with additional bands present Non-supportive: Weak band of predicted size but with additional bands of higher intensity also present Non-supportive: Weak band of predicted size but with additional bands of higher intensity also present 

         Protein array

Image

   

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
   

Antibody dilution

1:3000   

Validation PA

Supportive: Pass with single peak corresponding to interaction only with its own antigen.   
 

ANTIGEN VIEW

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E2F1-001