The normal tissue page shows antibody staining and, when available, knowledge-based annotated protein expression in 46 human tissues. The assay and annotation is described here.
This page starts with a summary. The summary data can be based on one or more antibodies (reported by the "Antibodies in assay"-field). If the data is based on one single antibody, the IH validation score for that antibody is reported in the "Reliability (Single)"-field (color-coding described here). If the data is based on more than one antibody, the reliability score for the data is reported as "Reliability (APE)" (color-coding described here).
The tissues and cell types can be ordered by organ, cell type or alphabetically.
For each tissue and cell type, the antibody staining is given with the yellow-scale color-coding (described by the scale in the box to the right). Each available antibody is listed in a separate column and the antibody identifier is available at the bottom of the tissue list. The images and annotations can be accessed by clicking on the tissue name.
When available, a knowledge-based annotated protein expression (APE) is given to the right of the antibody staining values. The level of expression is given by a blue-scale color-coding (described by the scale in the box to the right).
At the very bottom of this page, a summary of the staining from each antibody is given, together with a representative image and the immunohistochemistry validation score for the antibody.
Immunohistochemical staining of human testis shows strong nuclear positivity in cells of seminiferus ducts.
Immunohistochemical staining of human rectum shows strong nuclear positivity in glandular cells.
Immunohistochemical staining of human colon shows strong nuclear positivity in glandular cells.
Summary
Most normal tissues displayed moderate to strong nuclear positivity. Neuronal cells in CNS and islet cells in pancreas were negative.
Moderate to strong nuclear positivity was observed in most normal tissues. Cytoplasmic staining was observed in a few tissues including hepatocytes and stomach.
Most of the normal cells showed moderate to strong nuclear/nucleolar positivity, in some cases accompanied with an additional cytoplasmic staining. Islet cells of pancreas were weakly stained. Bile duct cells were negative.
Validation
Two (or more) antibodies yielding partly similar staining patterns which are consistent with gene/protein characterization data
One antibody yielding a staining pattern which is consistent with gene/protein characterization data
Two (or more) antibodies yielding partly similar staining patterns which are consistent with gene/protein characterization data
