Fields »

SUBCELLULAR LOCATION

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Main location(s)

Nucleus

Additional location(s)

Nucleoli 

Staining summary

Localized to the nucleus and nucleolus.

Reliability (APE)

Low

Antibodies in assay

CAB000329, CAB019308
 

Antibody CAB000329

 

Antibody CAB019308

 

         Cell line A-431

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Location(s)

Nucleus but not nucleoli Nucleus, Nucleoli 

Staining characteristics

  

Staining intensity

Weak Moderate 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data.
 
One/multiple location(s) supported by experimental gene/protein characterization data.
 

Comment

  

         Cell line U-2 OS

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Location(s)

 Nucleus, Nucleoli 

Staining characteristics

  

Staining intensity

Negative Strong 

Validation

No staining.
 
One/multiple location(s) supported by experimental gene/protein characterization data.
 

Comment

  

         Cell line U-251 MG

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Location(s)

Nucleus but not nucleoli Nucleus, Nucleoli 

Staining characteristics

  

Staining intensity

Weak Moderate 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data.
 
One/multiple location(s) supported by experimental gene/protein characterization data.
 

Comment

  

         Antibody summary


Immunofluorescent staining of human cell line U-251 MG shows positivity in nucleus but not nucleoli.
 

Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleus & nucleoli.
 

Summary

Staining of the nucleus in A-431 and U-251 MG.   Staining of nucleus in all three cell lines.  

Validation

The subcellular location is supported by literature.
 
The subcellular location is supported by literature.
 

Dictionary

Nucleoli
Nucleus