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SUBCELLULAR LOCATION

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Main location(s)

Cytoplasm

Additional location(s)

Nucleoli, Nucleus but not nucleoli, Centrosome 

Staining summary

Staining of cytoplasm and nucleus but not nucleoli in all three cell lines. Additional staining of the centrosomes in A-431 and U-251 MG.

Reliability (Single)

Antibodies in assay

HPA036374
 

Antibody HPA036374

 

         Cell line A-431

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Location(s)

Nucleoli, Cytoplasm, Centrosome 

Staining characteristics

 

Staining intensity

Moderate 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data.
 

Comment

 

         Cell line U-2 OS

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Location(s)

Nucleus but not nucleoli, Cytoplasm 

Staining characteristics

 

Staining intensity

Moderate 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data.
 

Comment

 

         Cell line U-251 MG

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Location(s)

Nucleus but not nucleoli, Cytoplasm, Centrosome 

Staining characteristics

Granular 

Staining intensity

Strong 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data.
 

Comment

 

         Antibody summary


Immunofluorescent staining of human cell line A-431 shows positivity in nucleoli, cytoplasm & centrosome.
 

Summary

Staining of cytoplasm and nucleus but not nucleoli in all three cell lines. Additional staining of the centrosomes in A-431 and U-251 MG.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 

Dictionary

Centrosome
Cytoplasm
Nucleoli
Nucleus but not nucleoli