Fields »

SUBCELLULAR LOCATION

? »

Main location(s)

Nucleus but not nucleoli, Cytoplasm

Additional location(s)

 

Staining summary

Localized to the nucleus and cytoplasm.

Reliability (APE)

High

Antibodies in assay

HPA003014, HPA003060, CAB004997
 

Antibody HPA003014

 

Antibody HPA003060

 

Antibody CAB004997

 

         Cell line A-431

Toggle channels

 
 
 

Location(s)

Nucleus but not nucleoli, Cytoplasm Nucleus but not nucleoli, Cytoplasm  

Staining characteristics

Granular Granular  

Staining intensity

Moderate Moderate Negative 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but supported by ≥1 other antibody.
 
One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but supported by ≥1 other antibody.
 
No staining.
 

Comment

   

         Cell line U-2 OS

Toggle channels

    
 

Location(s)

     

Staining characteristics

     

Staining intensity

    Negative 

Validation

    
No staining.
 

Comment

     

         Cell line U-251 MG

Toggle channels

 
 
 

Location(s)

Nucleus but not nucleoli, Cytoplasm Nucleus but not nucleoli, Cytoplasm  

Staining characteristics

Granular Granular  

Staining intensity

Weak Weak Negative 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but supported by ≥1 other antibody.
 
One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but supported by ≥1 other antibody.
 
No staining.
 

Comment

   

         Antibody summary


Immunofluorescent staining of human cell line A-431 shows positivity in nucleus but not nucleoli & cytoplasm.
 

Immunofluorescent staining of human cell line A-431 shows positivity in nucleus but not nucleoli & cytoplasm.
 

Immunofluorescent staining of human cell line U-2 OS shows no positivity.
 

Summary

Staining of nucleus and cytoplasm in A-431 and U-251 MG.   Staining of cytoplasm and nucleus in U-251MG and A-431.   No staining was observed in any of the three cell lines.  

Validation

The subcellular location is supported by literature.
 
The subcellular location is supported by literature.
 
The subcellular location is partly supported by literature or no literature is available.
 

Dictionary

Cytoplasm
Nucleus but not nucleoli