Fields »

SUBCELLULAR LOCATION

? »

Main location(s)

Nuclear membrane

Additional location(s)

Endoplasmic reticulum 

Staining summary

Mainly localized to the nuclear membrane. In addition localized to the endoplasmic reticulum.

Reliability (APE)

High

Antibodies in assay

HPA000609, CAB002029
 

Antibody HPA000609

 

Antibody CAB002029

 

         Cell line A-431

Toggle channels

 
 

Location(s)

Nuclear membrane, Endoplasmic reticulum Nuclear membrane 

Staining characteristics

  

Staining intensity

Strong Moderate 

Validation

Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 
One/multiple location(s) supported by experimental gene/protein characterization data.
 

Comment

  

         Cell line U-2 OS

Toggle channels

 
 

Location(s)

Nuclear membrane, Endoplasmic reticulum Nuclear membrane 

Staining characteristics

  

Staining intensity

Strong Weak 

Validation

Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 
One/multiple location(s) supported by experimental gene/protein characterization data.
 

Comment

  

         Cell line U-251 MG

Toggle channels

 
 

Location(s)

Nuclear membrane, Endoplasmic reticulum Nuclear membrane 

Staining characteristics

  

Staining intensity

Strong Weak 

Validation

Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 
One/multiple location(s) supported by experimental gene/protein characterization data.
 

Comment

  

         Antibody summary


Immunofluorescent staining of human cell line U-251MG shows positivity in nuclear membrane & endoplasmic reticulum.
 

Immunofluorescent staining of human cell line U-2 OS shows positivity in nuclear membrane.
 

Summary

Strong staining of nuclear membrane and slightly weaker staining of endoplasmic reticulum in all three cell lines.   Staining of nuclear membrane in all three cell lines.  

Validation

The subcellular location is supported by literature.
 
The subcellular location is supported by literature.
 

Dictionary

Endoplasmic reticulum
Nuclear membrane