Fields »

SUBCELLULAR LOCATION

? »

Main location(s)

Nucleus

Additional location(s)

Cytoplasm 

Staining summary

Mainly localized to the nucleus but also to the cytoplasm.

Reliability (APE)

Medium

Antibodies in assay

HPA029849, HPA029852
 

Antibody HPA029849

 

Antibody HPA029852

 

         Cell line A-431

Toggle channels

 
 

Location(s)

Nucleus but not nucleoli Nucleus 

Staining characteristics

 Spotty 

Staining intensity

Moderate Strong 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data.
 
One/multiple location(s) with no available experimental gene/protein characterization data.
 

Comment

  

         Cell line U-2 OS

Toggle channels

  
 

Location(s)

  Nucleus 

Staining characteristics

  Spotty 

Staining intensity

  Strong 

Validation

  
One/multiple location(s) with no available experimental gene/protein characterization data.
 

Comment

   

         Cell line U-251 MG

Toggle channels

 
 

Location(s)

 Nucleus but not nucleoli, Cytoplasm 

Staining characteristics

 Spotty 

Staining intensity

Negative Weak 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data.
 
One/multiple location(s) with no available experimental gene/protein characterization data.
 

Comment

  

         Antibody summary


Immunofluorescent staining of human cell line A-431 shows positivity in nucleus but not nucleoli.
 

Immunofluorescent staining of human cell line U-251MG shows positivity in nucleus but not nucleoli & cytoplasm.
 

Summary

Staining of nucleus in A-431.   Staining of nucleus in all three cell lines with additional staining of cytoplasm in U-251 MG.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is partly supported by literature or no literature is available.
 

Dictionary

Cytoplasm
Nucleus