Fields »

SUBCELLULAR LOCATION

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Main location(s)

Plasma membrane, Golgi apparatus

Additional location(s)

Cytoskeleton (Intermediate filaments) 

Staining summary

Localized to the plasma membrane or the Golgi apparatus.

Reliability (APE)

Very low

Antibodies in assay

HPA038782, HPA038783
 

Antibody HPA038782

 

Antibody HPA038783

 

         Cell line A-431

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Location(s)

Golgi apparatus Plasma membrane 

Staining characteristics

  

Staining intensity

Weak Moderate 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 
One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 

Comment

  

         Cell line U-2 OS

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Location(s)

Golgi apparatus Plasma membrane, Cytoskeleton (Intermediate filaments) 

Staining characteristics

  

Staining intensity

Moderate Strong 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 
One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 

Comment

  

         Cell line U-251 MG

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Location(s)

Golgi apparatus Plasma membrane 

Staining characteristics

  

Staining intensity

Weak Moderate 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 
One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 

Comment

  

         Antibody summary


Immunofluorescent staining of human cell line U-2 OS shows positivity in golgi apparatus.
 

Immunofluorescent staining of human cell line A-431 shows positivity in plasma membrane.
 

Summary

Staining of the Golgi apparatus in all three cell lines.   Staining of plasma membrane in all three cell lines with additional staining of cytoskeleton (intermediate filaments) in U-2OS.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is partly supported by literature or no literature is available.
 

Dictionary

Intermediate filaments
Golgi apparatus
Plasma membrane