MAGED2

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA031572

 

Antibody HPA031573

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 Atlas Antibodies
Sigma-Aldrich
 

Product name

HPA031572 HPA031573 

Host species

Rabbit Rabbit 

Clonality

pAb pAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand Affinity purified using the PrEST-antigen as affinity ligand 

Released in version

6 6 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment Recombinant protein fragment 

Length (aa)

88 81 

Antigen sequence

MSDTSESGAGLTRFQAEASEKDSSSMMQTLLTVTQNVEVPETPKASKALE
VSEDVKVSKASGVSKATEVSKTPEAREAPATQASSTTQ
 
LTDTQVLAAENKSLAADTKKQNADPQAVTMPATETKKVSHVADTKVNTKA
QETEAAPSQAPADEPEPESAAAQSQENQDTR
 

Matching transcripts

MAGED2-002 - ENSP00000364209 [100%]
MAGED2-003 - ENSP00000364198 [100%]
MAGED2-201 - ENSP00000218439 [100%]
MAGED2-202 - ENSP00000364193 [100%]
MAGED2-204 - ENSP00000379526 [100%]
MAGED2-004 - ENSP00000336962 [80%]
 MAGED2-002 - ENSP00000364209 [100%]
MAGED2-003 - ENSP00000364198 [100%]
MAGED2-004 - ENSP00000336962 [100%]
MAGED2-005 - ENSP00000364200 [100%]
MAGED2-201 - ENSP00000218439 [100%]
MAGED2-202 - ENSP00000364193 [100%]
MAGED2-203 - ENSP00000364202 [100%]
MAGED2-204 - ENSP00000379526 [100%]
 

Other gene match

  

ANTIBODY VALIDATION

         Immunohistochemistry

Image

  

Description

Immunohistochemical staining of human small intestine shows strong cytoplasmic and membranous positivity in glandular cells.
More information
 Immunohistochemical staining of human colon shows strong cytoplasmic positivity in glandular cells.
More information
 

Retrieval method

HIER pH6 HIER pH6 

Antibody dilution

1:30 1:350 

Literature conformity

No avaliable characterization data No avaliable characterization data 

RNA consistency

Not done Not done 

         Immunofluorescence

Image

  

Description

Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleus, nucleoli & cytoplasm.
More information
 Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleus, nucleoli & cytoplasm.
More information
 

Antibody dilution

1:57 1:200 

Validation IF

Uncertain: The subcellular location is partly supported by literature or no literature is available. Uncertain: The subcellular location is partly supported by literature or no literature is available. 

         Western Blot

Image

  

Description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: Negative control (vector only transfected HEK293T lysate)
Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY415179)
More information
 

Target mass (kDa)

65.0, 63.1 65.0, 63.1, 55.8 

Antibody dilution

1:250 1:250 

Validation WB

Supportive: Single band corresponding to the predicted size in kDa (+/-20%) Supportive: Single band corresponding to the predicted size in kDa (+/-20%) 

         Protein array

Image

  

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 

Antibody dilution

1:3000 1:3000 

Validation PA

Uncertain: Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%). Supportive: Pass with single peak corresponding to interaction only with its own antigen. 
 

ANTIGEN VIEW

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MAGED2-002
 
MAGED2-003
 
MAGED2-004
 
MAGED2-005
 
MAGED2-201
 
MAGED2-202
 
MAGED2-203
 
MAGED2-204