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SUBCELLULAR LOCATION

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Main location(s)

Plasma membrane

Additional location(s)

Nucleus but not nucleoli, Cytoplasm, Golgi apparatus 

Staining summary

Mainly localized to the plasma membrane but also detected in the cytoplasm, Golgi apparatus and plasma membrane.

Reliability (APE)

Medium

Antibodies in assay

HPA011740, HPA014290
 

Antibody HPA011740

 

Antibody HPA014290

 

         Cell line A-431

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Location(s)

Cytoplasm, Golgi apparatus Nucleus but not nucleoli, Plasma membrane 

Staining characteristics

  

Staining intensity

Moderate Moderate 

Validation

Not decisive - One/multiple location(s) where experimental gene/protein characterization data is partly supporting and partly conflicting.
 
Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 

Comment

  

         Cell line U-2 OS

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Location(s)

Plasma membrane, Cytoplasm Plasma membrane 

Staining characteristics

Granular  

Staining intensity

Weak Weak 

Validation

Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 
One/multiple location(s) supported by experimental gene/protein characterization data.
 

Comment

  

         Cell line U-251 MG

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Location(s)

Cytoplasm Nucleus but not nucleoli, Plasma membrane 

Staining characteristics

Granular  

Staining intensity

Moderate Moderate 

Validation

Not decisive - One/multiple location(s) where experimental gene/protein characterization data is partly supporting and partly conflicting.
 
Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 

Comment

  

         Antibody summary


Immunofluorescent staining of human cell line U-2 OS shows positivity in plasma membrane & cytoplasm.
 

Immunofluorescent staining of human cell line U-2 OS shows positivity in plasma membrane.
 

Summary

Staining of cytoplasm in all three cell lines with additional staining of Golgi apparatus in A-431 and plasma membrane in U-2 OS.   Staining of plasma membrane in all three cell lines with additional staining of nucleus in U-251 MG and A-431.  

Validation

The subcellular location is supported by literature.
 
The subcellular location is supported by literature.
 

Dictionary

Cytoplasm
Golgi apparatus
Nucleus but not nucleoli
Plasma membrane