Fields »

SUBCELLULAR LOCATION

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Main location(s)

Nucleus, Nuclear membrane, Cytoplasm

Additional location(s)

 

Staining summary

Localized to the nucleus, nuclear membrane and cytoplasm.

Reliability (APE)

Very low

Antibodies in assay

HPA039533, HPA040355
 

Antibody HPA039533

 

Antibody HPA040355

 

         Cell line A-431

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Location(s)

Nucleus but not nucleoli Nucleus, Nuclear membrane, Cytoplasm 

Staining characteristics

  

Staining intensity

Weak Moderate 

Validation

Location not consistent with experimental gene/protein characterization data.
 
Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 

Comment

  

         Cell line U-2 OS

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Location(s)

Nucleus Nucleus, Nuclear membrane, Cytoplasm 

Staining characteristics

  

Staining intensity

Weak Weak 

Validation

Location not consistent with experimental gene/protein characterization data.
 
Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 

Comment

  

         Cell line U-251 MG

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Location(s)

Cytoplasm Nucleus, Cytoplasm 

Staining characteristics

  

Staining intensity

Weak Moderate 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data.
 
Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 

Comment

  

         Antibody summary


Immunofluorescent staining of human cell line A-431 shows positivity in nucleus but not nucleoli.
 

Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleus, nuclear membrane & cytoplasm.
 

Summary

Staining of nucleus in A-431 and U-2 OS and cytoplasm in U-251 MG.   Staining of nucleus and cytoplasm in all three cell lines with additional staining of nuclear membrane in A-431 and U-2 OS:  

Validation

The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is supported by literature.
 

Dictionary

Cytoplasm
Nuclear membrane
Nucleus