Fields »

SUBCELLULAR LOCATION

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Main location(s)

Vesicles

Additional location(s)

Golgi apparatus, Centrosome 

Staining summary

Mainly localized to vesicles but also Golgi apparatus and centrosome.

Reliability (APE)

Medium

Antibodies in assay

HPA026488, HPA028425
 

Antibody HPA026488

 

Antibody HPA028425

 

         Cell line A-431

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Location(s)

Golgi apparatus, Vesicles, Centrosome Cytoplasm, Vesicles 

Staining characteristics

  

Staining intensity

Weak Moderate 

Validation

One/multiple location(s) in cytoplasm (e.g. golgi, mitochondria) supported by experimental evidence for cytoplasmic localization.
 
One/multiple location(s) with no available experimental gene/protein characterization data.
 

Comment

  

         Cell line U-2 OS

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Location(s)

Cytoplasm, Vesicles Cytoplasm, Vesicles 

Staining characteristics

Granular  

Staining intensity

Weak Strong 

Validation

One/multiple location(s) in cytoplasm (e.g. golgi, mitochondria) supported by experimental evidence for cytoplasmic localization.
 
One/multiple location(s) with no available experimental gene/protein characterization data.
 

Comment

  

         Cell line U-251 MG

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Location(s)

Centrosome Cytoplasm, Vesicles 

Staining characteristics

  

Staining intensity

Weak Strong 

Validation

Not decisive - One/multiple location(s) where experimental gene/protein characterization data is partly supporting and partly conflicting.
 
One/multiple location(s) with no available experimental gene/protein characterization data.
 

Comment

  

         Antibody summary


Immunofluorescent staining of human cell line A-431 shows positivity in golgi apparatus, vesicles & centrosome.
 

Immunofluorescent staining of human cell line U-2 OS shows positivity in cytoplasm & vesicles.
 

Summary

Staining of centrosome in U-251 MG and A-431 with additional staining of Golgi and vesicles in A-431. Staining of cytoplasm in U-2 OS.   Staining of cytoplasm and vesicles in all three cell lines.  

Validation

The subcellular location is supported by literature.
 
The subcellular location is partly supported by literature or no literature is available.
 

Dictionary

Centrosome
Golgi apparatus
Endosome