Fields »

SUBCELLULAR LOCATION

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Main location(s)

Cytoplasm

Additional location(s)

Nucleus but not nucleoli, Vesicles 

Staining summary

Localized to cytoplasm, nucleus and vesicles.

Reliability (APE)

Very low

Antibodies in assay

CAB000458, HPA003524
 

Antibody CAB000458

 

Antibody HPA003524

 

         Cell line A-431

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Location(s)

 Cytoplasm 

Staining characteristics

  

Staining intensity

Negative Moderate 

Validation

No staining.
 
Location not consistent with experimental gene/protein characterization data.
 

Comment

  

         Cell line U-2 OS

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Location(s)

 Vesicles 

Staining characteristics

  

Staining intensity

Negative Moderate 

Validation

No staining.
 
Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 

Comment

  

         Cell line U-251 MG

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Location(s)

 Nucleus but not nucleoli, Cytoplasm 

Staining characteristics

 Granular 

Staining intensity

Negative Moderate 

Validation

No staining.
 
Location not consistent with experimental gene/protein characterization data.
 

Comment

  

         Antibody summary


Immunofluorescent staining of human cell line A-431 shows no positivity.
 

Immunofluorescent staining of human cell line U-2 OS shows positivity in vesicles.
 

Summary

No staining was observed in any of the three cell lines.   Staining of vesicles in U-2 OS. Staining of cytoplasm and nucleus in U-251 MG. Staining of cytoplasm in A-431.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is partly supported by literature or no literature is available.
 

Dictionary

Cytoplasm
Nucleus but not nucleoli
Endosome