The normal tissue page shows antibody staining and, when available, knowledge-based annotated protein expression in 46 human tissues. The assay and annotation is described here.
This page starts with a summary. The summary data can be based on one or more antibodies (reported by the "Antibodies in assay"-field). If the data is based on one single antibody, the IH validation score for that antibody is reported in the "Reliability (Single)"-field (color-coding described here). If the data is based on more than one antibody, the reliability score for the data is reported as "Reliability (APE)" (color-coding described here).
The tissues and cell types can be ordered by organ, cell type or alphabetically.
For each tissue and cell type, the antibody staining is given with the yellow-scale color-coding (described by the scale in the box to the right). Each available antibody is listed in a separate column and the antibody identifier is available at the bottom of the tissue list. The images and annotations can be accessed by clicking on the tissue name.
When available, a knowledge-based annotated protein expression (APE) is given to the right of the antibody staining values. The level of expression is given by a blue-scale color-coding (described by the scale in the box to the right).
At the very bottom of this page, a summary of the staining from each antibody is given, together with a representative image and the immunohistochemistry validation score for the antibody.
Immunohistochemical staining of human liver shows strong cytoplasmic positivity in hepatocytes.
Immunohistochemical staining of human kidney shows strong cytoplasmic positivity with a granular pattern in tubular cells.
Immunohistochemical staining of human rectum shows strong cytoplasmic positivity in glandular cells.
Summary
Moderate to strong cytoplasmic positivity with a granular pattern was observed in most normal tissues. Cardiac muscle, hepatocytes, renal tubules, parietal cells in gastric glands, testicular cells and the intestine displayed strong immunoreactivity. Squamous epithelia, renal glomeruli and bile duct cells were negative.
Normal tissues showed moderate to strong granular cytoplasmic staining. Bile duct cells, most cells in CNS and renal glomeruli showed weak positivity or were negative.
Most of the normal tissues displayed moderate granular cytoplasmic positivity. Strong staining was observed in the gastro-intestinal tract, hepatocytes, renal tubules, urothelium and the adrenal cortex. Alveolar cells, bile duct cells, ovarian and endometrial stroma was negative.
Validation
Two (or more) antibodies yielding similar staining patterns which are consistent with available gene/protein characterization data
Two (or more) antibodies yielding similar staining patterns which are consistent with available gene/protein characterization data
Two (or more) antibodies yielding similar staining patterns which are consistent with available gene/protein characterization data
