LAPTM4B

ANTIBODY AND ANTIGEN INFORMATION

? »
 

Antibody HPA014996

 

Antibody CAB020782

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 SDIX
 

Product name

HPA014996 3156.00.02 

Host species

Rabbit Rabbit 

Clonality

pAb msAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand Affinity 

Released in version

4 5 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment Genetic immunization 

Length (aa)

49  

Antigen sequence

NCYRYINGRNSSDVLVYVTSNDTTVLLPPYDDATVNGAAKEPPPPYVSA
 
 

Matching transcripts

LAPTM4B-001 - ENSP00000402301 [100%]
LAPTM4B-002 - ENSP00000428409 [100%]
  

Other gene match

  

ANTIBODY VALIDATION

         Immunofluorescence

Image

  

Description

Immunofluorescent staining of human cell line A-431 shows positivity in nucleus but not nucleoli & vesicles.
More information
  

Antibody dilution

1:42  

Validation IF

Uncertain: The subcellular location is partly supported by literature or no literature is available.  

         Western Blot

Image

  

Description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT-4
Lane 3: U-251 MG
Lane 4: Kidney
Lane 5: Liver
Lane 6: Tonsil
More information
  

Target mass (kDa)

35.1, 25.4 35.1, 25.4, 23.1 

Antibody dilution

1:250 1:500 

Validation WB

Supportive: Single band corresponding to the predicted size in kDa (+/-20%) Non-supportive: Weak band of predicted size but with additional bands of higher intensity also present 

         Protein array

Image

  

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
  

Antibody dilution

1:3000  

Validation PA

Supportive: Pass with single peak corresponding to interaction only with its own antigen.  

         Immunohistochemistry

Image

  

Description

 Immunohistochemical staining of human colon shows strong positivity in glandular cells.
More information
 

Retrieval method

 HIER pH6 

Antibody dilution

 1:90 

Validation IH

 Uncertain: One antibody yielding a staining pattern which is partly consistent with gene/protein characterization data or consistent with limited gene/protein characterization data 
 

ANTIGEN VIEW

? »
 
 
 
LAPTM4B-001
 
LAPTM4B-002