VRK3

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA016040

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 

Product name

HPA016040 

Host species

Rabbit 

Clonality

pAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand 

Released in version

4 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment 

Length (aa)

116 

Antigen sequence

TTSLEALPTGTVLTDKSGRQWKLKSFQTRDNQGILYEAAPTSTLTCDSGP
QKQKFSLKLDAKDGRLFNEQNFFQRAAKPLQVNKWKKLYSTPLLAIPTCM
GFGVHQDKYRFLVLPS
 

Matching transcripts

VRK3-001 - ENSP00000324636 [100%]
VRK3-002 - ENSP00000366210 [100%]
VRK3-004 - ENSP00000472541 [100%]
VRK3-005 - ENSP00000471061 [100%]
VRK3-006 - ENSP00000470156 [100%]
VRK3-007 - ENSP00000473171 [100%]
VRK3-008 - ENSP00000469710 [100%]
VRK3-009 - ENSP00000469880 [100%]
 

Other gene match

 

ANTIBODY VALIDATION

         Immunohistochemistry

Image

 

Description

Immunohistochemical staining of human spleen shows strong positivity in granulocytes.
More information
 

Retrieval method

HIER pH6 

Antibody dilution

1:150 

Validation IH

Uncertain: One antibody yielding a staining pattern and there is no available gene/protein characterization data or the staining pattern is partly consistent with or contradicted by limited gene/protein characterization data 

         Western Blot

Image

 

Description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT-4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 

Target mass (kDa)

52.9, 47.5, 45.9, 45.2, 40.5 

Antibody dilution

1:250 

Validation WB

Supportive: Band of predicted size in kDa (+/-20%) with additional bands present 

         Protein array

Image

 

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 

Antibody dilution

1:3000 

Validation PA

Supportive: Pass with single peak corresponding to interaction only with its own antigen. 
 

ANTIGEN VIEW

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VRK3-001
 
VRK3-002
 
VRK3-004
 
VRK3-005
 
VRK3-006
 
VRK3-007
 
VRK3-008
 
VRK3-009