Fields »

SUBCELLULAR LOCATION

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Main location(s)

Plasma membrane, Cytoplasm

Additional location(s)

Nucleus but not nucleoli 

Staining summary

Mainly localized to the cytoplasm and plasma membrane. Additionally localized to the nucleus.

Reliability (APE)

Medium

Antibodies in assay

HPA024305, CAB040563
 

Antibody HPA024305

 

Antibody CAB040563

 

         Cell line A-431

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Location(s)

Plasma membrane, Cytoplasm Plasma membrane, Cytoplasm 

Staining characteristics

  

Staining intensity

Strong Moderate 

Validation

Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 
Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 

Comment

  

         Cell line U-2 OS

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Location(s)

Nucleus but not nucleoli, Plasma membrane, Cytoplasm Plasma membrane, Cytoplasm 

Staining characteristics

  

Staining intensity

Moderate Strong 

Validation

Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 
Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 

Comment

  

         Cell line U-251 MG

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Location(s)

Nucleus but not nucleoli, Plasma membrane, Cytoplasm Plasma membrane, Cytoplasm 

Staining characteristics

  

Staining intensity

Moderate Strong 

Validation

Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 
Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 

Comment

  

         Antibody summary


Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleus but not nucleoli, plasma membrane & cytoplasm.
 

Immunofluorescent staining of human cell line A-431 shows positivity in plasma membrane & cytoplasm.
 

Summary

Staining of cytoplasm and plasma membrane in all three cell lines with additional staining of the nucleus in U-251 MG and U-2 OS.   Staining of cytoplasm, nucleus and plasma membrane in all three cell lines.  

Validation

The subcellular location is supported by literature.
 
The subcellular location is supported by literature.
 

Dictionary

Cytoplasm
Nucleus but not nucleoli
Plasma membrane