The normal tissue page shows antibody staining and, when available, knowledge-based annotated protein expression in 46 human tissues. The assay and annotation is described here.
This page starts with a summary. The summary data can be based on one or more antibodies (reported by the "Antibodies in assay"-field). If the data is based on one single antibody, the IH validation score for that antibody is reported in the "Reliability (Single)"-field (color-coding described here). If the data is based on more than one antibody, the reliability score for the data is reported as "Reliability (APE)" (color-coding described here).
The tissues and cell types can be ordered by organ, cell type or alphabetically.
For each tissue and cell type, the antibody staining is given with the yellow-scale color-coding (described by the scale in the box to the right). Each available antibody is listed in a separate column and the antibody identifier is available at the bottom of the tissue list. The images and annotations can be accessed by clicking on the tissue name.
When available, a knowledge-based annotated protein expression (APE) is given to the right of the antibody staining values. The level of expression is given by a blue-scale color-coding (described by the scale in the box to the right).
At the very bottom of this page, a summary of the staining from each antibody is given, together with a representative image and the immunohistochemistry validation score for the antibody.
Immunohistochemical staining of human rectum shows strong cytoplasmic and membranous positivity in glandular cells.
Immunohistochemical staining of human rectum shows strong cytoplasmic positivity in glandular cells. Microvilli also displayed strong immunoreactivity.
Immunohistochemical staining of human duodenum shows strong cytoplasmic positivity in a subset of leukocytes.
Immunohistochemical staining of human appendix shows strong cytoplasmic and membranous positivity in glandular cells.
Summary
Strong cytoplasmic staining was observed in glandular epithelia of the GI tract as well as mucosal squamous epithelia. Remaining normal tissues were in general negative.
Gastro-intestinal tract, hepatocytes, squamous epithelia, endometrial, rep pulp in spleen and prostate glands displayed moderate to strong cytoplasmic and/or membranous positivity. Endocrine glands, exocrine pancreas, CNS, lymphoid tissues and mesenchymal cells were generally negative.
Weak to moderate cytoplasmic and membranous staining was observed in intercalated ducts of salivary gland, microvilli in glandular epithelia of the gastrointestinal tract and in trophoblastic cells in placenta. Subsets of leukocytes were strongly stained in several cases. Remaining normal tissues were in general negative.
Most normal tissues were in general negative. Strong membranous and cytoplasmic positivity was seen in fraction of glandular cells of colon, rectum and appendix.
Validation
Two (or more) antibodies yielding similar staining patterns which are consistent with available gene/protein characterization data
Two (or more) antibodies yielding similar staining patterns which are consistent with available gene/protein characterization data
The multi-targeting antibody yielding a staining pattern partly consistent with gene/protein characterization data or consistent with limited gene/protein characterization data for all of the genes
Two (or more) antibodies yielding similar staining patterns which are consistent with available gene/protein characterization data
