PTN

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA054601

 

Antibody CAB017685

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 Santa Cruz Biotechnology
 

Product name

HPA054601 sc-74443 

Host species

Rabbit Mouse 

Clonality

pAb mAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand Protein A/G 

Released in version

12 4 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment Not known 

Length (aa)

64  

Antigen sequence

KQFGAECKYQFQAWGECDLNTALKTRTGSLKRALHNAECQKTVTISKPCG
KLTKPKPQAESKKK
 
 

Matching transcripts

PTN-001 - ENSP00000341170 [100%]
PTN-002 - ENSP00000376798 [92%]
  

Other gene match

  

ANTIBODY VALIDATION

         Immunohistochemistry

Image

  

Description

 Immunohistochemical staining of human testis shows strong cytoplasmic positivity in subsets of cells in ductus seminiferus.
More information
 

Retrieval method

 HIER pH6 

Antibody dilution

 1:80 

Validation IH

 Uncertain: One antibody yielding a staining pattern which is partly consistent with gene/protein characterization data or consistent with limited gene/protein characterization data 

         Western Blot

Image

  

Description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT-4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT-4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 

Target mass (kDa)

18.9 18.9 

Antibody dilution

1:110 1:500 

Validation WB

Uncertain: No bands detected Uncertain: No bands detected 

         Immunofluorescence

Image

  

Description

Immunofluorescent staining of human cell line A-431 shows no positivity.
More information
  

Antibody dilution

1:22  

Validation IF

Uncertain: The subcellular location is partly supported by literature or no literature is available.  

         Protein array

Image

  

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
  

Antibody dilution

1:850  

Validation PA

Supportive: Pass with single peak corresponding to interaction only with its own antigen.  
 

ANTIGEN VIEW

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PTN-001
 
PTN-002