Fields »

SUBCELLULAR LOCATION

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Main location(s)

Cytoplasm

Additional location(s)

Nucleus, Golgi apparatus 

Staining summary

Mainly localized to the cytoplasm but also detected in the Golgi apparatus and nucleus.

Reliability (APE)

Low

Antibodies in assay

HPA013329, HPA026829
 

Antibody HPA013329

 

Antibody HPA026829

 

         Cell line A-431

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Location(s)

  Nucleus but not nucleoli, Cytoplasm 

Staining characteristics

   

Staining intensity

  Strong 

Validation

  
One/multiple location(s) with no available experimental gene/protein characterization data.
 

Comment

   

         Cell line U-2 OS

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Location(s)

Cytoplasm Nucleus, Nucleoli, Cytoplasm 

Staining characteristics

  

Staining intensity

Weak Strong 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data.
 
One/multiple location(s) with no available experimental gene/protein characterization data.
 

Comment

  

         Cell line U-251 MG

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Location(s)

Cytoplasm, Golgi apparatus Nucleus but not nucleoli, Cytoplasm 

Staining characteristics

Granular  

Staining intensity

Weak Strong 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data.
 
One/multiple location(s) with no available experimental gene/protein characterization data.
 

Comment

  

         Antibody summary


Immunofluorescent staining of human cell line U-251MG shows positivity in cytoplasm & golgi apparatus.
 

Immunofluorescent staining of human cell line A-431 shows positivity in nucleus but not nucleoli & cytoplasm.
 

Summary

Staining of cytoplasm in U-251 and U-2 OS with additional staining of Golgi apparatus in U-251 MG.   Staining of cytoplasm and nucleus in all three cell lines with additional staining of nucleoli in U-251 MG.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is partly supported by literature or no literature is available.
 

Dictionary

Cytoplasm
Golgi apparatus
Nucleus