Fields »

SUBCELLULAR LOCATION

? »

Main location(s)

Nucleus but not nucleoli, Cytoplasm

Additional location(s)

 

Staining summary

Localized to nuclear speckles and the cytoplasm.

Reliability (APE)

Medium

Antibodies in assay

HPA028064, HPA028516
 

Antibody HPA028064

 

Antibody HPA028516

 

         Cell line A-431

Toggle channels

 
 

Location(s)

 Nucleus but not nucleoli, Cytoplasm 

Staining characteristics

 Speckle 

Staining intensity

Negative Moderate 

Validation

No staining.
 
Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 

Comment

  

         Cell line U-2 OS

Toggle channels

 
 

Location(s)

 Nucleus but not nucleoli, Cytoplasm 

Staining characteristics

 Speckle 

Staining intensity

Negative Strong 

Validation

No staining.
 
Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 

Comment

  

         Cell line U-251 MG

Toggle channels

 
 

Location(s)

 Nucleus but not nucleoli, Cytoplasm 

Staining characteristics

 Speckle 

Staining intensity

Negative Strong 

Validation

No staining.
 
Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 

Comment

  

         Antibody summary


Immunofluorescent staining of human cell line U-2 OS shows no positivity.
 

Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleus but not nucleoli & cytoplasm.
 

Summary

No staining was observed in any of the three cell lines.   Staining of cytoplasm and speckled nucleus in all three cell lines.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is supported by literature.
 

Dictionary

Cytoplasm
Nucleus but not nucleoli