Fields »

SUBCELLULAR LOCATION

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Main location(s)

Nucleus but not nucleoli

Additional location(s)

Vesicles 

Staining summary

Mainly localized to the nucleus but also to vesicles.

Reliability (APE)

Low

Antibodies in assay

HPA008435, HPA024230
 

Antibody HPA008435

 

Antibody HPA024230

 

         Cell line A-431

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Location(s)

Nucleus but not nucleoli Nucleus but not nucleoli 

Staining characteristics

Granular  

Staining intensity

Weak Moderate 

Validation

Not decisive - One/multiple location(s) where experimental gene/protein characterization data is partly supporting and partly conflicting.
 
Not decisive - One/multiple location(s) where experimental gene/protein characterization data is partly supporting and partly conflicting.
 

Comment

  

         Cell line U-2 OS

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Location(s)

Nucleus but not nucleoli Nucleus but not nucleoli, Vesicles 

Staining characteristics

Granular  

Staining intensity

Weak Weak 

Validation

Not decisive - One/multiple location(s) where experimental gene/protein characterization data is partly supporting and partly conflicting.
 
Not decisive - One/multiple location(s) where experimental gene/protein characterization data is partly supporting and partly conflicting.
 

Comment

  

         Cell line U-251 MG

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Location(s)

Nucleus but not nucleoli Nucleus but not nucleoli, Vesicles 

Staining characteristics

Granular  

Staining intensity

Weak Moderate 

Validation

Not decisive - One/multiple location(s) where experimental gene/protein characterization data is partly supporting and partly conflicting.
 
Not decisive - One/multiple location(s) where experimental gene/protein characterization data is partly supporting and partly conflicting.
 

Comment

  

         Antibody summary


Immunofluorescent staining of human cell line U-251MG shows positivity in nuclei but not nucleoli.
 

Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleus but not nucleoli & vesicles.
 

Summary

Staining of nuclei but not nucleoli in all three cell lines.   Staining of nucleus in all three cell lines. Additional staining of vesicles in U-251 MG and U-2 OS.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is partly supported by literature or no literature is available.
 

Dictionary

Nucleus but not nucleoli
Endosome