Fields »

SUBCELLULAR LOCATION

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Main location(s)

Cytoplasm

Additional location(s)

Nucleus, Nucleoli, Centrosome 

Staining summary

Localized to the cytoplasm.

Reliability (APE)

High

Antibodies in assay

CAB025196, HPA030419, HPA030420
 

Antibody CAB025196

 

Antibody HPA030419

 

Antibody HPA030420

 

         Cell line A-431

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Location(s)

  Cytoplasm 

Staining characteristics

  Fibrous 

Staining intensity

Negative Negative Moderate 

Validation

No staining.
 
No staining.
 
One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but supported by ≥1 other antibody.
 

Comment

   

         Cell line U-2 OS

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Location(s)

 Cytoplasm Cytoplasm 

Staining characteristics

   

Staining intensity

Negative Weak Weak 

Validation

No staining.
 
One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but supported by ≥1 other antibody.
 
One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but supported by ≥1 other antibody.
 

Comment

   

         Cell line U-251 MG

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Location(s)

 Cytoplasm Cytoplasm 

Staining characteristics

   

Staining intensity

Negative Weak Weak 

Validation

No staining.
 
One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but supported by ≥1 other antibody.
 
One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but supported by ≥1 other antibody.
 

Comment

   

         Antibody summary


Immunofluorescent staining of human cell line U-2 OS shows no positivity.
 

Immunofluorescent staining of human cell line U-251 MG shows positivity in cytoplasm.
 

Immunofluorescent staining of human cell line A-431 shows positivity in cytoplasm.
 

Summary

No staining was observed in any of the three cell lines.   Staining of cytoplasm in U-251 MG and U-2 OS.   Staining of cytoplasm in all three cell lines.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is supported by literature.
 
The subcellular location is supported by literature.
 

Dictionary

Centrosome
Cytoplasm
Nucleoli
Nucleus