The normal tissue page shows antibody staining and, when available, knowledge-based annotated protein expression in 46 human tissues. The assay and annotation is described here.
This page starts with a summary. The summary data can be based on one or more antibodies (reported by the "Antibodies in assay"-field). If the data is based on one single antibody, the IH validation score for that antibody is reported in the "Reliability (Single)"-field (color-coding described here). If the data is based on more than one antibody, the reliability score for the data is reported as "Reliability (APE)" (color-coding described here).
The tissues and cell types can be ordered by organ, cell type or alphabetically.
For each tissue and cell type, the antibody staining is given with the yellow-scale color-coding (described by the scale in the box to the right). Each available antibody is listed in a separate column and the antibody identifier is available at the bottom of the tissue list. The images and annotations can be accessed by clicking on the tissue name.
When available, a knowledge-based annotated protein expression (APE) is given to the right of the antibody staining values. The level of expression is given by a blue-scale color-coding (described by the scale in the box to the right).
At the very bottom of this page, a summary of the staining from each antibody is given, together with a representative image and the immunohistochemistry validation score for the antibody.
Immunohistochemical staining of human nasopharynx shows strong cytoplasmic positivity in respiratory epithelial cells.
Immunohistochemical staining of human urinary bladder shows strong cytoplasmic positivity in a subset of urothelial cells.
Immunohistochemical staining of human small intestine shows moderate positivity in glandular cells.
Summary
Most normal tissues displayed weak to moderate cytoplasmic immunoreactivity. Placental trophoblasts and a subset of cells in red pulp of spleen demonstrated strong positivity. Alveolar cells, non-germinal lymphoid cells and cell in liver were mainly negative.
Most normal tissues showed weak to moderate cytoplasmic and/or membranous staining. Strong staining was observed in respiratory epithelia, salivary gland,
stomach, subsets of cells in urinary bladder, trophoblastic cells in placenta and seminal vesicle. Lymphoid tissues were generally negative.
Normal glandular cells showed moderate cytoplasmic immunoreactivity while most other cell types were weakly stained or negative. Exocrine pancreas, placental trophoblasts, adrenal cortical cells and Leydig cells revealed strong positivity.
Validation
Two (or more) antibodies yielding similar or partly similar staining patterns and there is no available gene/protein characterization data
Two (or more) antibodies yielding similar or partly similar staining patterns and there is no available gene/protein characterization data
Two (or more) antibodies yielding similar or partly similar staining patterns and there is no available gene/protein characterization data
